Apis mellifera L. males (drones) and reproductive females (queens) exhibit specific mating times during the day. Since their arrival in 1994, Africanized honey bees represent the local population on the island of Puerto Rico, and there is a small apicultural sector on the island that uses European bees. In an island, bee races can show greater rate of hybridization because of the genetic isolation that exists from mainland. ¶¶We predicted that Africanized genes are diluted in the local populations because of previous crosses with various European races in mainland and current crosses in the island. In addition, the current influx of European queens increases the male population that could mate with Africanized queens. We examined rate of hybridization by use of molecular markers. To test current mating hypothesis, two apiaries were used, each one with four Africanized and four European beehives. ¶We marked the drones as they emerged from pupal cells and return to their colonies.¶ W¶e compared the mating flight time of Africanized queens and European and Africanized drones. We also examined drift of drones across colonies of both races, indicating a common pool of drones for mating. ¶¶To determine the parental origin or race of the source colonies we analyzed the drones by examining mitochondrial DNA using RFLP techniques.¶ In previous analysis of drones, we found a mix of races inside colonies.