In order to test whether the ants form supercolonies we sampled colonies from various localities and measured their intra-and interspecific aggression as well as intra-colony variations in cuticular hydrocarbon profiles. Preliminary chemical analyses followed by principle components analysis showed that nests from different localities possess distinctive profiles. However, using a group encounter paradigm for assessing the ants’ aggression, the ants didn’t show intraspecific aggression even when nests from extremes of their local distribution were tested. Interspecific aggression was ubiquitously high and generally culminated in the heterospecific ants’ death. It is still not clear whether W. auropunctata form supercolonies in Israel, as it is known to form in other countries of its invasive range. Preliminary observations suggest that where the little fire ant density is high, no other ant species are present, while in areas of low density or no fire ants, up to 15 other ant species occur; we are currently analyzing ant community structure in a gradient of little fire ant densities.
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